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fusx assembly kit  (Addgene inc)
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Addgene inc fusx assembly kit
Schematic diagram of FusXTBE pairs used in this protocol and <t>their</t> <t>assembly</t> (A) Golden Gate cloning of <t>FusX</t> library enables the rapid synthesis of FusXTBE clones. (B) Each FusXTBE arm consists of a DNA binding domain with either 15 or 16-RVDs targeting 15 or 16-nucleotides DNA binding sequence, which is preceded by a 5′ T nucleotide. Attached to this module is a split half of the DddA tox protein and an UGI molecule. The 14–18 bp protospacer region in between two arms are amenable for deaminase activity. Both the figures were created by using Biorender.com .
Fusx Assembly Kit, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Schematic diagram of FusXTBE pairs used in this protocol and their assembly (A) Golden Gate cloning of FusX library enables the rapid synthesis of FusXTBE clones. (B) Each FusXTBE arm consists of a DNA binding domain with either 15 or 16-RVDs targeting 15 or 16-nucleotides DNA binding sequence, which is preceded by a 5′ T nucleotide. Attached to this module is a split half of the DddA tox protein and an UGI molecule. The 14–18 bp protospacer region in between two arms are amenable for deaminase activity. Both the figures were created by using Biorender.com .

Journal: STAR Protocols

Article Title: An optimized FusX assembly-based technique to introduce mitochondrial TC-to-TT variations in human cell lines

doi: 10.1016/j.xpro.2022.101288

Figure Lengend Snippet: Schematic diagram of FusXTBE pairs used in this protocol and their assembly (A) Golden Gate cloning of FusX library enables the rapid synthesis of FusXTBE clones. (B) Each FusXTBE arm consists of a DNA binding domain with either 15 or 16-RVDs targeting 15 or 16-nucleotides DNA binding sequence, which is preceded by a 5′ T nucleotide. Attached to this module is a split half of the DddA tox protein and an UGI molecule. The 14–18 bp protospacer region in between two arms are amenable for deaminase activity. Both the figures were created by using Biorender.com .

Article Snippet: The Ekker Lab’s FusX assembly kit can be ordered through Addgene (Kit No. 1000000063).

Techniques: Cloning, Clone Assay, Binding Assay, Sequencing, Activity Assay

Journal: STAR Protocols

Article Title: An optimized FusX assembly-based technique to introduce mitochondrial TC-to-TT variations in human cell lines

doi: 10.1016/j.xpro.2022.101288

Figure Lengend Snippet:

Article Snippet: The Ekker Lab’s FusX assembly kit can be ordered through Addgene (Kit No. 1000000063).

Techniques: Recombinant, Plasmid Preparation, Gel Extraction, Purification, Sequencing, Amplification, Software, Membrane, Pore Size, Spectrophotometry, Microscopy